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Wydział Biotechnologii i Nauk o Żywności PŁ

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Zainteresowania naukowe i realizowana tematyka badań

Metodyka badań krystalograficznych, krystalizacja białek i pomiary dyfrakcyjne kryształów,
Krystalografia białek i związków organicznych,
Komplementarność metod NMR ciała stałego i krystalografii

prof. dr hab. inż

Grzegorz

Bujacz

Stanowisko

profesor

Funkcja

kierownik Zespołu Krystalografii Białek, członek Rady Dyscypliny Nauki Chemiczne

E-mail

grzegorz.bujacz@p.lodz.pl

Telefon

+48 42-631-34-31

Pokój

301

ORCID

0000-0002-3787-7522

ResearcherID

https://publons.com/researcher/4446-2018

SCOPUS

6603986318

Publikacje po 2010 roku

Pawlak T., Sudgen I., Bujacz G., Iuga D., Brown S.P., Potrzebowski M.J. Synergy of Solid-State NMR, Single-Crystal X-ray Diffraction, and Crystal Structure Prediction Methods: A Case Study of Teriflunomide (TFM). Cryst Growth Des. 2021, 21:3328–3343.
Bujacz A., Rum J., Rutkiewicz M., Pietrzyk-Brzezinska A.J., Bujacz G. Structural Evidence of Active Site Adaptability towards Different Sized Substrates of Aromatic Amino Acid Aminotransferase from Psychrobacter Sp. B6. Materials 2021, 14, 3351, 2-19.
Khalaji M., Wroblewska A., Wielgus E., Bujacz G.D., Dudek M.K., Potrzebowski M.J. Structural variety of heterosynthons in linezolid cocrystals with modified thermal properties. Acta Crystallogr B Struct Sci Cryst Eng Mater. 2020;76:892–912.
Leszczynska G., Cypryk M., Gostynski B., Sadowska K., Herman P., Bujacz, G. Lodyga-Chruscinska E., Sochacka E., Nawrot B. C5-Substituted 2-Selenouridines Ensure Efficient Base Pairing with Guanosine; Consequences for Reading the NNG-3 ` Synonymous mRNA Codons. Int J Mol Sci. 2020, 21(8):2882.
Pawlak T., Czajkowska-Szczykowska D., Jastrzebska I., Santillan R., Seroka B., Maj J., Morzycki J.W., Labra-Vazquez P., Farfan N., Bujacz G.D., Potrzebowski M.J. Influence of Hydrogen/Fluorine Substitution on Structure, Thermal Phase Transitions, and Internal Molecular Motion of Aromatic Residues in the Crystal Lattice of Steroidal Rotors. Cryst Growth Des. 2020, 20:2202–2216.
Wroblewska A., Sniechowska J., Kazmierski S., Wielgus E., Bujacz G.D. Mloston G., Chworos A., Suwara J., Potrzebowski M.J. Application of 1-Hydroxy-4,5-Dimethyl-Imidazole 3-Oxide as Coformer in Formation of Pharmaceutical Cocrystals. Pharmaceutics 2020, 12(4):359.
Dudek M.K., Wielgus E., Paluch P., Sniechowska J., Kostrzewa M., Day G.M., Bujacz G.D., Potrzebowski M.J. Understanding the formation of apremilast cocrystals. Acta Crystallogr B Struct Sci Cryst Eng Mater. 2019, 75:803–814.
Rutkiewicz M., Bujacz A., Bujacz G. Structural features of cold-adapted dimeric GH2 beta-D-galactosidase from Arthrobacter sp. 32cB. Biochim Biophys Acta Proteins Proteom. 2019, 1867:776–786.
Orlikowska M., Rostro-Alanis M. de J., Bujacz A., Hernandez-Luna C., Rubio R., Parra R., Bujacz G. Structural studies of two thermostable laccases from the white-rot fungus Pycnoporus sanguineus. Int J Biol Macromolecules 2018, 107:1629–1640.
Sniechowska J., Paluch P., Pawlak T., Bujacz G.D., Danikiewicz W., Potrzebowski M.J. New synthetic pathway leading to oxospirochlorins. RSC Adv. 2018, 8:21354–21362.
Wroblewska A., Paluch P., Wielgus E., Bujacz G., Dudek M.K., Potrzebowski M.J. Approach toward the Understanding of Coupling Mechanism for EDC Reagent in Solvent-Free Mechanosynthesis. Org Lett. 2017, 19:5360–5363.
Dudek M.K., Bujacz G., Potrzebowski M.J. Experimental tests for quality validation of computationally predicted crystal structures - a case of a conformationally flexible procyanidin A-2 dihydrate. CrystEngComm. 2017, 19:2903–2913.
Blauz A., Rychlik B., Makal A., Szulc K., Strzelczyk P., Bujacz G., Zakrzewski J., Wozniak K., Plazuk D. Ferrocene-Biotin Conjugates: Synthesis, Structure, Cytotoxic Activity and Interaction with Avidin. ChemPlusChem 2016, 81:1191–1201.
Strzelczyk P., Plazuk D., Zakrzewski J., Bujacz G. Structural Characterization of the Avidin Interactions with Fluorescent Pyrene-Conjugates: 1-Biotinylpyrene and 1-Desthiobiotinylpyrene. Molecules 2016, 21(10):1270.
Strzelczyk P., Bujacz G. Crystal structure and ligand affinity of avidin in the complex with 4’-hydroxyazobenzene-2-carboxylic acid. J Mol Struct. 2016, 1109:232–238.
Orlikowska M., Liberek K., Bujacz G. Crystallization of heat shock protein essential for protein disaggregation. Acta Crystallogr A-Foundation Adv 2016, 72:S247.
Sliwiak J., Dolot R., Michalska K., Szpotkowski K., Bujacz G., Sikorski M., Jaskolski M. Crystallographic and CD probing of ligand-induced conformational changes in a plant PR-10 protein. J Struct Biol. 2016, 193:55–66.
Sniechowska J., Paluch P., Bujacz G., Gorecki M., Frelek J., Gryko D.T., Potrzebowski M.J., Chiral crystals from porphyrinoids possessing a very low racemization barrier. CrystEngComm. 2016, 18:3561–3565.
Strzelczyk P., Bujacz G.D., Kielbasinski P., Blaszczyk J. Crystal and molecular structure of hexagonal form of lipase B from Candida antarctica. ACTA Biochim Pol. 2016, 63:103–109.
Orlikowska M., Bujacz G. The structural studies of the two thermostable laccases from the white-rot fungi Pycnoporus sanguineus. Protein Sci. 2015, 24:253–254.
Jastrzebska K., Maciaszek A., Dolot R., Bujacz G., Guga P. Thermal stability and conformation of antiparallel duplexes formed by P-stereodefined phosphorothioate DNA/LNA chimeric oligomers with DNA and RNA matrices. Org Biomol Chem. 2015, 13:10032–10040.
Lukasik B., Mikolajczyk M., Bujacz G., Zurawinski R., Synthesis and the absolute configuration of both enantiomers of 4,5-dihydroxy-3-( formyl)-cyclopent-2-enone acetonide as a new chiral building block for prostanoid synthesis. Org Biomol Chem. 2015, 13:807–816.
Pietrzyk A.J., Bujacz A., Łochynska M., Jaskolski M., Bujacz G. Crystal Structure of Bombyx mori Lipoprotein 6: Comparative Structural Analysis of the 30-kDa Lipoprotein Family. PLoS ONE 2014, 9(11): e108761
Pawlak T., Paluch P., Jeziorna A., Bujacz G.D., Potrzebowski M.J. Slow and Very Fast MAS Solid State NMR Study of Biopolymers. Macromol Symp. 2014, 339:60–69.
Pietrzyk A.J., Panjikar S., Bujacz A., Lochynska M., Jaskolski M., Bujacz G. High-resolution structure of Bombyx mori lipoprotein 7: crystallographic determination of the identity of the protein and its potential role in detoxification. Acta Cryst. D Biol Crys. 2012, 68: 1140-1151.
Pietrzyk A.J., Bujacz A., Mueller-Dieckmann J., Łochynska M., Jaskolski M., Bujacz G. Two Crystal Structures of Bombyx mori Lipoprotein 3-Structural Characterization of a New 30-kDa Lipoprotein Family Member. PLoS ONE 2013, 8(4): e61303.
Pietrzyk A.J., Jaskolski M., Bujacz G. Structural studies of juvenile hormone binding proteins. [w] Juvenile Hormones and Juvenoids - Taylor & Francis Group, Edited By James Devillers e-book 2013 . DOI: 10.1201/b14899 - regural book 2018 by CRC Press.
Plazuk D., Zakrzewski J., Salmain M., Blauz A., Rychlik B., Strzelczyk P., Bujacz A., Bujacz G., Ferrocene-Biotin Conjugates Targeting Cancer Cells: Synthesis, Interaction with Avidin, Cytotoxic Properties and the Crystal Structure of the Complex of Avidin with a Biotin-Linker-Ferrocene Conjugate. Organometallics 2013, 32:5774–5783.
Dolot R., Wlodarczyk A., Bujacz G.D., Nawrot B. Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human histidine triad nucleotide-binding protein 2 (hHINT2). ACTA Crystallogr Sect F-STRUCTURAL Biol Commun 2013, 69:783–787.
Strzelczyk P., Bujacz A., Plazuk D., Zakrzewski J., Bujacz G., Structural investigation of the interactions of biotinylruthenocene with avidin. Chem Biol Interact. 2013, 204:6–12.
Pietrzyk A.J., Panjikar S., Bujacz A., Mueller-Dieckmann J., Lochynska M., Jaskolski M., Bujacz G., High-resolution structure of Bombyx mori lipoprotein 7: Crystallographic determination of the identity of the protein and its potential role in detoxification. Acta Crystallogr Sect D Biol Crystallogr. 68: 1140-1151.
Ziolkowska N.E., Bujacz A., Randad R.S., Erickson J.W., Skalova T., Hasek J., Bujacz G. New Active HIV-1 Protease Inhibitors Derived from 3-Hexanol: Conformation Study of the Free Inhibitors in Crystalline State and in Complex with the Enzyme. Chem Biol Drug Des. 2012, 79:798–809.
Pietrzyk A.J., Bujacz A., Lochyska M., Jaskolski M., Bujacz G. Isolation, purification, crystallization and preliminary X-ray studies of two 30 kDa proteins from silkworm haemolymph. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011, 67:372–376.
Trzeciak-Karlikowska K., Bujacz A., Ciesielski W., Bujacz G.D., Potrzebowski M.J. The Influence of the Stereochemistry of Alanine Residue on the Solid State Conformation and Crystal Packing of Opioid Peptides Containing D-Ala or L-Ala in Message Domain - XRD and NMR Study. J Phys Chem B 2011, 115:9910–9919.
Bujacz A., Jedrzejczak-Krzepkowska M., Bielecki S., Redzynia I., Bujacz G. Crystal structures of the apo form of beta-fructofuranosidase from Bifidobacterium longum and its complex with fructose. FEBS J 2011, 278:1728–1744.
Belghith Y., Belkhiria M.S., Bujacz G., Bujacz A., Nasri H. Structural Characterization of the {[}Co-II(TPP)(18-C-6)(2)] Complex. Acta Crystallogr A-Foundation Adv 2011, 67:C219.
Nowicka K., Bujacz A., Paluch P., Sobczuk A., Jeziorna A., Ciesielski W., Bujacz G.D., Jurczak J., Potrzebowski M.J. Study of host-guest interactions in benzodiazacoronands by means of solid state NMR spectroscopy, X-ray diffraction and quantum mechanical computations. Phys Chem Chem Phys. 2011, 13:6423–6433.
Cakici O., Sikorski M., Stepkowski T., Bujacz G., Jaskolski M. Crystal Structures of NodS N-Methyltransferase from Bradyrhizobium japonicum in Ligand-Free Form and as SAH Complex. J Mol Biol. 2010, 404:874–889.
Bujacz G., Wrzesniewska B., Bujacz A. Cryoprotection properties of salts of organic acids: a case study for a tetragonal crystal of HEW lysozyme. ACTA Crystallogr Sect D-STRUCTURAL Biol. 2010, 66:789–796.
Ziolkowska N.E., Michejda C.J., Bujacz G.D. Structural studies of series HIV-1 nonnucleoside reverse transcriptase inhibitors 1-(2,6-difluorobenzyl)-2-(2,6-difluorophenyl)-benzimidazoles with different 4-substituents. J Mol Struct. 2010, 966:53–58.
Ziolkowska N.E., Michejda C.J., Bujacz G.D. Structure of HIV-1 nonnucleoside reverse transcriptase inhibitors derivatives of N-benzyl-benzimidazole with different substituents in position 4. J Mol Struct. 2010, 963:188–193.
Koclega K.D., Chruszcz M., Zimmerman M.D., Bujacz G., Minor W., ``Hot’’ Macromolecular Crystals. Cryst Growth Des. 2010, 10:580–586.

Projekty (zrealizowane i realizowane oraz pełniona w nich rola) po 2010 roku:

Bujacz G. (wykonawca), N N405 363939, Badania strukturalne albumin: surowicy wołowej (BSA) i ludzkiej (HSA) – analiza oddziaływań z ligandami, 2010-2013.
Bujacz G. (kierownik), 2011/03/B/NZ1/01238, Proteomika strukturalna hemolimfy jedwabnika morwowego, 2012-2015.
Bujacz G. (wykonawca), 2013/11/B/ST5/02271, Badania krystalograficzne ssaczych surowiczych albumin w kompleksach z ligandami, 2013-2016.
Bujacz A. (wykonawca), 2016/21/B/ST5/00555, Badania strukturalne enzymów zimnolubnych - mechanizm przystosowania do zimna, 2017-2019.