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Scientific activities ITB

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Research topics at the Institute of Technical Biochemistry

Industrial Biochemistry

Biocatalysis

  • Enzymes application in plant biomass conversion: optimization of various industrial by-products and wastes of plant origin enzymatic saccharificationconditions
  • Characterization of hydrolysates and residues from hydrolysis in terms of chemical composition and usage in fermentation processes
  • Application of enzymes in enantioselective conversion of chemical compounds
  • Identification and quantification of selected chemical compounds, e.g. glucose and other monosaccharides, alcohols and organic acids using specific enzymatic tests

Biotransformations

  • Biosynthesis of various microbial enzymes, including glycoside hydrolases (cellulases, pectinases, chitosanases, amylases), triacylglycerol lipases and proteases in submerged and solid statecultures
  • Production of enzymatic preparations, including immobilization of enzymes and cells
  • Interactions of microbial enzymes with carbon nanotubes and graphene
  • Optimization of conditions of agri-foodwastes (containing carbohydrates, fats and proteins) enzymatic conversion into value-added products
  • Biotransformationsin non-aqueous media using immobilized biocatalysts (cells and enzymes)
  • Biosynthesis and conversions of lipid substances
  • Biotransformations of chitosan into industriallyusefulcompounds

Bioremediation

  • Brown coal biosolubilization
  • Bioremediation of soil contaminated with crude oil-based products (diesel oil, transformer oil, pyrolysis oil, fractions derived by crude oil distillation)
  • Innovative bioremediation technologies (enzyme preparations, sorbents, biosurfactants)
  • Monitoring of bioremediation processes based on biochemical and physicochemical parameters
  • Biodegradation of waste materials (rubber)
  • Isolation, screening and application in bioremediation of endophytes
  • Biodegradation of biodiesel

Molecular biotechnology

Taxonomic identification of microorganisms on the basis of molecular biology techniques

  • Finding homologous sequences of DNA and phylogenetic trees creation

Recombinant DNA technology in vitro

  • Isolation and analysis of nucleic acids ofdifferent origins (plasmid, chromosomal and metagenomic DNA and RNA of microorganisms)
  • Microbial gene libraries construction
  • PCR and sequencing
  • Restriction analysis of DNA fragments, performing restriction maps
  • Expression of recombinant DNA  in bacteria and yeast

Bioinformatic analysis of the DNA sequence of entire microbial genomes

  • Assembly of contigs from short sequences, scaffolding
  • Comperative genomics
  • Sequence genome annotation through homology of known sequences, discovery of new genes

Proteomics

  • Analysis of the protein profiles of wild-type microorganisms and their mutants using two dimensional gel electrophoresis (2D)
  • Identification of differentiating proteins using appropriate computer software, e.g. PDQueast

Protein engineering

  • Selection and analysis of mutations in the target protein on the basis of sequence alignments and structural alignments with template proteins,
  • Application of protein engineering methods in enzyme design(directed evolution, site-directed and site-saturation mutagenesis)
  • Application of homologous modelling and in silico docking in protein design

Bacterial nanocellulose

Application and molecular aspects of biosynthesis

  • The mechanism of Gluconacetobacter  xylinus motility and its influence on bionanocellulose structure
  • The Gluconacetobacter  xylinus bionanocellulose synthesis mechanism and its regulation (genetic and bioinformatics studies)
  • Identification of Gluconacetobacter xylinus genes involved in bionanocellulose biosynthesis
  • Expression, purification and characterization of  Gluconacetobacter xylinus selected proteins
  • Bionanocellulose biosynthesis intensification and structure modification in termsof novel biomedical and industrial applications 

Proteomics/Structural biology

  • Structural studies of biologically active compounds  for the medical or biotechnological purposes – biomolecules crystallization, crystallographic studies of native proteins and their complexes with ligands as well as peptides and synthetic small molecules
  • Crystal structure determination basing on diffraction experiment performed on monocrystals
  • Protein-ligand interaction analysis, e.g. enzyme-inhibitor, antibody-ligand, protein-protein, protein-nucleic acid
  • Crystallographic studies for determination of protein structures that are potential therapeutic targets
  • Determining properties of the biotechnologically useful proteins basing on structural data
  • Usage of crystallography and solid state NMR as complementary techniques in structural studies of biomolecules
  • The development of crystallographic methods: optimization of crystallization process,   synchrotron measurements, and derivative technique for measuring protein with MAD and SAD procedures

Biochemistry of food and nutrigenomics

  • Characteristics of raw materials and industrial fruit and vegetable products in terms of qualitative and quantitative contents of vitamins, plant dyes, amino acids, organic acids and polyphenolic compounds
  • Defining of antioxidative activity of standard substances and antioxidative potential of food in vitro (ABTS, DPPH, FRAP, TBAR’s)
  • Assessment of food ingredients stability under conditions of in vitro simulated digestion
  • Assessment of polyphenolic compounds and digestive enzymes interactions
  • Characteristics of pro-health activity of vegetable and fruit ingredients (polyphenols, glucosinolates, isothiocyanates, terpenes) and synthetic compounds in vitro (cell cultures) with special focus to metabolic syndrome and other diseases of affluence
  • Assessment of cytotoxicity, efficiency and molecular basics of activity of chemotherapeutics against various cancer cell lines
  • Performance of biocompatibility test in terms of cell toxicity in vitro
  • Studies on methods promoting neovascularization within chronic wounds – the therapeutic angiogenesis

The Institute of Technical Biochemistry Collection of Microorganisms 

Contact: Aneta Białkowska, PhD. (aneta.bialkowska@p.lodz.pl; tel. +48 42 631 34 29)


Faculties